APHARMAS -

Description:

We specialize in constructing high-quality cDNA library from a variety of cells and tissues for general applications and special microarray. Our team has 15+ years experience with 100+ libraries constructed from,

Advantages:

• Rigorous mRNA QC steps to test ribosomal RNA and genomic DNA contamination
• High quality size-fractionated large insert cDNA library
• Choice of vector for library construction
• Low vector background and high percentage of recombinants
• Large number of primary clones
• Library transformation into T1 phage resistant E. coli
• Custom vector and protocol acceptable

Standard cDNA Library Construction

• 3~5 x 10⁶ primary clones
• Average insert size 1~1.5 kb with > 85% recombinants
• Size-selected libraries are offered
• 15 random clones are tested by PCR or enzyme digestion for QC
• Library provided in glycerol or DMSO

Normalized cDNA Libraries

• Normalization is carried out at various Cot levels (Cot₁₀, Cot₅₀ or Cot₅₀₀) according to relative abundance to genes that needs to be normalized
• Percent Normalization is tested by:
  • Relative PCR for house keeping genes and low abundant genes
  • Colony/plaque hybridization of 3,000 ~ 30,000 clones before and after normalization
  • DNA sequencing of 96 random cDNA clones
• Guarantee to provide 1x10⁶ primary clones
• Two size selected cDNA libraries are provided (0.5 ~ 2 kb and 2+ Kb)
• Average inserts size at least 1~1.5 kb and over 85% recombinants
• Libraries can be made directionally and in a vector of your need

Subtracted cDNA Libraries

• Subtraction is carried out according to relative abundance to genes that needs to be subtracted
• Percent Subtraction are tested by:
  • Relative PCR for house keeping genes and low abundant genes
  • Colony/plaque hybridization of 3,000~ 30,000 clones before and after subtraction
  • DNA sequencing of 96 random cDNA clones
• Guarantee to provide 1x10⁶ primary clones
• Two size selected cDNA libraries are provided (0.5 ~ 2 kb and 2+ Kb)
• Average inserts size of at least 1~1.5 kb and over 90% recombinants
• Libraries can be made directionally and in a vector of your need

For more technical details and cost information, please This e-mail address is being protected from spambots. You need JavaScript enabled to view it .