APHARMAS:

Description:

This service is based on advantage of lentiviral vectors that can stably deliver shRNA into primary cells or cell lines with up to 100% efficiency. Our Lenti-Ready^sm Technology provides higher efficiency and more flexibility to meet customers' needs. Ready-to-use deliverables are suitable for in vitro and/or in vivo applications.

If commercial shRNAs or siRNAs of your target gene are not available or non-functional, or your project requires special design and/or IP-protection for further development, we meet your needs!

Advantages:

• One-stop service: from shRNA design to packaging
• Extremely high infection efficiency
• Quick turnaround time: 4 to 6 weeks
• Cost-effective
• Special design request acceptable, FREE!

Requirements & Deliverables:

Only GenBank accession number or a DNA sequence is needed.

Deliverables:

√ Ready-to-use viral supernatants
√ Infection protocol

Options:
* Choice of selection marker: Blasticidin or GFP or your marker;
* Constitutive or inducible;
* Concentration for in vivo application;
* Packaging-only if you already have a construct;
* Re-order of additional volume at reduced cost.
* Control virus: vector-only or scrambled shRNA

Service Documents (Registered User ONLY):

• General service information (Login)

Reference:

1. Terashima, A, etc. An Essential Role for PICK1 in NMDA Receptor-Dependent Bidirectional Synaptic Plasticity. Neuron. 2008 March 27; 57(6): 872-882.
2. Harwood, FC, etc. mTORC1 Signaling Can Regulate Growth Factor Activation of p44/42 Mitogen-activated Protein Kinases through Protein Phosphatase 2A. J. Biol. Chem. 283, 2575-2585.
3. Sha et al. A Critical Role for CHIP in the Aggresome Pathway.Molecular and Cellular Biology, January 2009, p. 116-128, Vol. 29, No. 1
4. Monroe et al.  Retinoblastoma binding protein-1(RBP1) is a Runx2 coactivator and promotes osteoblastic differentiation. BMC Musculoskeletal Disorders 2010, 11:104

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