APHARMAS:

Description:

We offers protein purification services for cDNA cloned into bacterial, mammalian or insect cells. Recombinant protein production is a core activity involved in high throughput screening functional proteins for validation and structural biology. This service is a complete solution for your customized needs that we partner with your research team working side by side.

Most customers use this service for laboratory application. If you need large-scale protein expression, please visit our "bioproduction" section.

1. Assessment and Optimization of Expression

• Expression of proteins are optimized by testing various cloning vectors and host cells
• Time course study of expression to minimize protein degradation and proper folding
• Small scale protein purification and QC by SDS-PAGE and western blot analysis

2. Protein Purification

• Large-scale growth of over expressing construct and cells
• Harvesting cells and cell lysis by sonication or homoginization
• Protein purification on affinity tag resins
• Secondary purification on anion exchange resin or by gel filtration
• Dialysis and western blot analysis
• Vialing and packaging of proteins

For more technical details and cost information, please This e-mail address is being protected from spambots. You need JavaScript enabled to view it.