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Protein Purification Services

 

gene expressionDescription:

We offers protein purification services for cDNA cloned into bacterial, mammalian or insect cells. Recombinant protein production is a core activity involved in high throughput screening functional proteins for validation and structural biology. This service is a complete solution for your customized needs that we partner with your research team working side by side.

Most customers use this service for laboratory application. If you need large-scale protein expression, please visit our "bioproduction" section.

 

1. Assessment and Optimization of Expression

  • Expression of proteins are optimized by testing various cloning vectors and host cells
  • Time course study of expression to minimize protein degradation and proper folding
  • Small scale protein purification and QC by SDS-PAGE and western blot analysis

 

2. Protein Purification

  • Large-scale growth of over expressing construct and cells
  • Harvesting cells and cell lysis by sonication or homoginization
  • Protein purification on affinity tag resins
  • Secondary purification on anion exchange resin or by gel filtration
  • Dialysis and western blot analysis
  • Vialing and packaging of proteins

 

For more technical details and cost information, please This e-mail address is being protected from spambots. You need JavaScript enabled to view it.

Cat. #
Project Name
Price
4008
Custom Protein Expression Optimization Service This e-mail address is being protected from spambots. You need JavaScript enabled to view it.
4009 Custom Protein Purification Service This e-mail address is being protected from spambots. You need JavaScript enabled to view it.

 

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