APHARMAS:

Description:

In addition to lentivirus-meidated gene expression service, we also provide this service in a variety of expression systems such as prokaryotic, eukaryotic, and baculovirus system to meet your needs. The selection of expression system is based on your preference with our optimized protocol.

Advantages:

• Significant cost-saving over DIY
• Experienced technical staff and professional project management
• Quick turnaround time with high throughput capacity

What we do:

• Preparation and purification of recombinant vector for transfection
• Transfection of cDNA into mammalian/ insect/ bacterial cells
• Selection of over expression cells under optimized conditions
• Western blot analysis of the expression
• If required,
  • Large scale growth of over-expression cells
  • Preparation and purification of recombinant proteins and QC by Western blot

Options:

• One stop service from cloning to expression:
  • Isolate a gene of your interest from a supplied vector and subclone it into a plasmid or baculovirus transfer vector
  • Verify recombinant plasmid or baculovirus transfer vector by restriction mapping and DNA sequencing
  • Complete above expression services
• Characterization of a protein with mass spectrometry, immunology, cellular behavior and enzyme activity.
• Production process optimization

For more technical details and a quote, please This e-mail address is being protected from spambots. You need JavaScript enabled to view it.