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cDNA Library Construction Service

 

cdna library

Description:

We specialize in constructing high-quality cDNA libraries from a variety of cells and tissues for general applications and special microarray. Our technical team has 15+ years experience with 100+ libraries constructed from,

  • Human
  • Mouse
  • Rat
  • Monkey
  • Plant
  • Algae
  • Bacteria
  • Fungi
  • Yeast
  • Fruit fly
  • Protozoa
  • Fish...

Advantages:

  • Rigorous mRNA QC steps to test ribosomal RNA and genomic DNA contamination
  • High quality size-fractionated large insert cDNA library
  • High percentage of recombinants and large number of primary clones
  • Library transformation into T1 phage resistant E. coli
  • Custom vector and protocol acceptable
  • Cost-effective and quick turn around time

 

Standard cDNA Library

  • > 1 x 106 primary clones
  • Average insert size > 1 kb with > 85% recombinant rate
  • Size-selected libraries are offered
  • 15 random clones are tested by PCR or enzyme digestion for QC
  • Library provided in glycerol or DMSO

Normalized cDNA Library

  • Normalization is carried out at various Cot levels (Cot10, Cot50 or Cot500) according to relative abundance to genes that needs to be normalized
  • Percent Normalization is tested by:
    • Relative PCR for house keeping genes and low abundant genes
    • Colony/plaque hybridization of 3,000 ~ 30,000 clones before and after normalization
    • DNA sequencing of 96 random cDNA clones
  • Guarantee to provide > 1x106 primary clones
  • Average inserts size > 1 kb and over 85% recombinant rate
  • Libraries can be made directionally in a vector of your choice

Subtractive cDNA Library

  • Subtraction is carried out according to relative abundance to genes that needs to be subtracted
  • Percent Subtraction are tested by:
    • Relative PCR for house keeping genes and low abundant genes
    • Colony/plaque hybridization of 3,000~ 30,000 clones before and after subtraction
    • DNA sequencing of 96 random cDNA clones
  • Guarantee to provide 1x106 primary clones
  • Average inserts size > 1 kb and over 85% recombinant rate
  • Libraries can be made directionally in a vector of your choice

 

For more technical details and a quote, please This e-mail address is being protected from spambots. You need JavaScript enabled to view it. .

Cat. #
Project Name
Price
2001
Custom Standard cDNA Library Construction Service This e-mail address is being protected from spambots. You need JavaScript enabled to view it.
2002
Custom Normalized cDNA Library Construction Service This e-mail address is being protected from spambots. You need JavaScript enabled to view it.
2003 Custom Subtracted cDNA Library Construction Service This e-mail address is being protected from spambots. You need JavaScript enabled to view it.

 

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