APHARMAS:

Description:

We specialize in constructing high-quality cDNA libraries from a variety of cells and tissues for general applications and special microarray. Our technical team has 15+ years experience with 100+ libraries constructed from,

Advantages:

• Rigorous mRNA QC steps to test ribosomal RNA and genomic DNA contamination
• High quality size-fractionated large insert cDNA library
• High percentage of recombinants and large number of primary clones
• Library transformation into T1 phage resistant E. coli
• Custom vector and protocol acceptable
• Cost-effective and quick turn around time

Standard cDNA Library

• > 1 x 10⁶ primary clones
• Average insert size > 1 kb with > 85% recombinant rate
• Size-selected libraries are offered
• 15 random clones are tested by PCR or enzyme digestion for QC
• Library provided in glycerol or DMSO

Normalized cDNA Library

• Normalization is carried out at various Cot levels (Cot₁₀, Cot₅₀ or Cot₅₀₀) according to relative abundance to genes that needs to be normalized
• Percent Normalization is tested by:
  • Relative PCR for house keeping genes and low abundant genes
  • Colony/plaque hybridization of 3,000 ~ 30,000 clones before and after normalization
  • DNA sequencing of 96 random cDNA clones
• Guarantee to provide > 1x10⁶ primary clones
• Average inserts size > 1 kb and over 85% recombinant rate
• Libraries can be made directionally in a vector of your choice

Subtractive cDNA Library

• Subtraction is carried out according to relative abundance to genes that needs to be subtracted
• Percent Subtraction are tested by:
  • Relative PCR for house keeping genes and low abundant genes
  • Colony/plaque hybridization of 3,000~ 30,000 clones before and after subtraction
  • DNA sequencing of 96 random cDNA clones
• Guarantee to provide 1x10⁶ primary clones
• Average inserts size > 1 kb and over 85% recombinant rate
• Libraries can be made directionally in a vector of your choice

For more technical details and a quote, please This e-mail address is being protected from spambots. You need JavaScript enabled to view it. .